Chelsea head to the game following a 2-0 victory over West Ham United in the Premier League encounter on Monday.
The Blues reached the Europa League last eight after defeating Dynamo Kiev 5-0 in the round of 16 second-leg encounter last month.
Below is the Chelsea squad against Slavia Praha:
Kepa Arrizabalaga, Willy Caballero, Marcin Bulka, Toni Rudiger, Andreas Christensen, Cesar Azpilicueta, David Luiz, Marcos Alonso, Emerson Palmieri, Davide Zappacosta, Jorginho, N’Golo Kante, Ross Barkley, Mateo Kovacic, Ruben Loftus-Cheek, Callum Hudson-Odoi, Eden Hazard, Pedro, Willian, Olivier Giroud, Gonzalo Higuain.
The kick-off time for the match is 8pm.